Shahed University

In vitro Shoot Multiplication of Haplophyllum virgatum var. Virgatum Axillary Bud Explants

Mahboubeh Abedi | Farah Karimi | Morteza Yousefzadi

Date :  2019/04/24
Publish in :    هشتمين کنگره ملي گياهان دارويي

Keywords :Shoot, Haplophyllum, virgatum

Abstract :
Plants belong to the genus Haplophyllum A. Juss. (Rutaceae), with 70 species, exist in warm, temperate and subtropical zones of earth including Eurasia and the tropical zone of northeast Africa. So far, the phytochemistry and medicinal properties of some of these species have been examined by analysing the contents of their essential oils, alkaloids, lignanes, glycosides and flavonoids, etc. 1, 2. For plant in vitro propagation, shoot multiplication through axillary bud culture would be the most suitable choice for obtaining vigorous shoots 3. Haplophyllum virgatum var. virgatum is one of the endemic varieties of the species which have limited to Geno region-Hormozgan province-Iran, so likely can categorized as an endangered species, its microropagation has not yet been studied. Here, the effects of different concentrations of BAP (6-Benzylaminopurine), a plant cytokinin, employed in vitro on axillary bud explants for shoot multiplication. The seeds were surface sterilized and cultured on B5 medium for explants preparation. Axillary bud explants obtained from weakly produced plantlets and were cultured in MS medium supplemented with BAP in total concentrations of 0.25, 0.5 and 0.75 mg/L, and incubated in a growth chamber with 25 °C temperature, 16 h photoperiod and a photon flux density of 400 μmol m-2 S-1. The most shoot multiplication observed in 0.75 mg/L BAP. The study on more BAP concentrations and the other kinds of plant kinetins are continued in our Lab.

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