Effect of Gamma Radiation on Production of Anticancer Drug Taxol and Antioxidant Potential in Hazelnut (Corylus avellana L.) Tissue Culture

Razieh Adabi | Ayatollah Rezaei

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=116589
Date :  2019/04/24
Publish in :    هشتمين کنگره ملي گياهان دارويي

Link :  http://www.ncmp2019.com/fa/
Keywords :Production, Taxol, Hazelnut, Corylus, avellana

Abstract :

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Plant cell culture is now recognized as one of the alternative and renewable sources for the production of secondary metabolites 1. Useful compounds of plant cell cultures are often secondary metabolites, which usually accumulate in very small amounts in plant cells. The use of elicitors can increase the production of secondary metabolites in cell cultures. Therefore, in this study, the effect of gamma radiation (0, 10, 20, 30 Gy) on hazelnut leaf derived callus culture was investigated in a completely randomized design with 4 replications. The parameters measured were callus growth, activity of catalase, peroxidase, polyphenol oxidase and phenylalanine ammonia lyase, antioxidant potential and taxol production. The results showed that the effect of gamma radiation with the exception of peroxidase activity on other parameters was significant. By increasing the dose of gamma radiation, the activity of the catalase, polyphenol oxidase and phenylalanine ammonia lyase enzymes increased significantly and at 30 Gy their activity was peaked. The production of taxol and antioxidant potential also increased significantly with increasing gamma radiation dose. The maximum amount of taxol measuserd at the . It seems that gamma radiation stimulated defence responses of cells and increased the production of secondary metabolites.

http://www.ncmp2019.com/fa/