Myelin enhancement of Multiple sclerosis model with gold nanoparticles into the corpus callosum

Mahjabin Khosravi | Manizheh Karami | Mohammadreza Jalali Nadoushan | Abazar Hajnorouzi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=116770
Date :  2019/08/26
Publish in :    Nanomedicine Journal
DOI :  https://doi.org/10.22038/nmj.2019.06.0002
Link :  http://nmj.mums.ac.ir/article_13308.html
Keywords :Keywords Corpus callosum, Demyelination, Gold nanoparticle, L-Arginine; Multiple sclerosis, Nitric oxide, Rat

Abstract :

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ABSTRACTAbstract Objective(s): With no substantial cost, we injected L-arginine into the rat’s corpus callosum (CC) to create animal model of multiple sclerosis (MS) and investigated the pre-injection effect of gold nanoparticles (GNPs). Materials and Methods: Adult male Wistar rat (250-300 g) was surgically cannulated at the CC, and after recovery it was injected L-arginine (3-200 µg/rat, intra-CC) once daily for 3 to 5 consecutive days. GNPs (0.001-0.01 µg/rat, intra-CC) were injected alone or prior to the L-arginine using the same procedure. Control group solely received saline (1 µL/rat, intra-CC). Brain was studied with luxol fast blue. Weight change was also analyzed via the analysis of variance (ANOVA). Results: L-arginine significantly induced (p 0.05) a reduction in the fiber density while the neurons increased (p 0.05). Single GNPs reduced (p 0.05) the fiber and neuron densities; however, pre-injection of NPs caused myelinated fibers and uniform density of neurons. Conclusion: The L-arginine may trigger demyelination by pro-inflammatory nitric oxide (NO), and the GNPs may improve this effect.