Shahed University

Lactuca scariola as Provider of Alkaloids to Make Molecularly Imprinted Detection Matrix

Shahpour Khangholi | Zahra Karimi Bekr | Soroush Sardari

Date :  2019/04/24
Publish in :    هشتمين کنگره ملي گياهان دارويي

Keywords :Alkaloids, MIP, Hyoscyamine

Abstract :
Alkaloids are a category of naturally occurring organic compounds that mostly contain fundamental nitrogen atoms. Alkaloids have a large range of pharmacological activities antihyperglycemic, antiasthma, anticancer and including antimalarial activities. Hyoscyamine is a tropane alkaloid. It is the levorotary isomer of atropine and thus sometimes known as levo-atropine. A molecularly imprinted polymer (MIP) is a polymer that has been processed using the molecular imprinting technique which leaves cavities in the polymer matrix with an affinity for a chosen template molecule. These polymers have dependency for the original molecule and have been used in usage such as chemical separations, catalysis, or molecular sensors 1. Lactuca scariola L. is a common weedy yearlong species found in disturbed and ruderal sites. It is a cosmopolitan species, at times prevailing in communities (Mucina, 1978), and is found at mid-latitudes all over Europe, Britain, North Africa, West Asia, and North America 2. Based on molecularly imprinted photonic polymer (MIP) that composed the colloidal-crystal with molecular imprintingmethod, a novel label-free colorimetric chemosensor for convenient and fast efficient detection of hyoscyamine with great sensitivity and specificity was developed. The plant was collected from Firouz-Abad, Fars Province in 2019. The voucher specimen was deposited in Drug Design Unit Herbarium. Different parts of the plant were collected and the extract was prepared using percolation. The unique three-dimensional, highlyordered photonic hydrogels would be obviously bound in response to the specific atropine molecular recognition process and the response would be directly transferred into visually perceptible optical signal (change in color) that could be detected by the naked eye. The intelligent chemosensor was successfully employed to assign the trace plane hyoscyamine in plant sample, providing a quick and impressive alternative for semi quantitative detection of hyoscyamine.

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