Shahed University

Peripheral blood mononuclear cellular viability and its correlation with long-term pulmonary complications after sulfur mustard exposure

Faramarz Fallahiarezoudar | Ali Mohamad Mohseni Majd | Ensie Sadat Mirsharif | Mohamad Reza Sedighi Moghadam | Razieh Dilmaghanian | Tooba Ghazanfari | Shams Jalaaldin

URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=116974
Date :  2019/10/13
Publish in :    International Immunopharmacology
DOI :  https://doi.org/10.1016/j.intimp.2019.105814
Link :  http://dx.doi.org/10.1016/j.intimp.2019.105814
Keywords :Long-term complications; Sulfur mustard; Vital activity

Abstract :
INTRODUCTION: Sulfur mustard (SM) as a chemical warfare agent has short- and long-term complications on its victims. Complications of exposure to SM depend on the level of contamination. Long-term pulmonary complications are the most serious problems. Recent evidence has shown that absorbed SM can be conducted to other tissues by the bloodstream. In this study, we evaluated the long-term effects of SM on the vital activity of peripheral blood mononuclear cells (PBMCs) in SM-exposed patients with long-term pulmonary complications. MATERIALS AND METHODS: Our study samples were 110 patients with long-term pulmonary complications in the SM-exposed group and 109 unexposed individuals in the control group. After clinical examination and pulmonary function tests, the severity of pulmonary complications was classified. Also, the participants peripheral blood was taken into EDTA-treated Vacutainer tubes. Then, the complete blood count (CBC) was calculated, and PBMCs was purified from whole blood using Ficol-Paque gradient method, finally, the vital activity was assessed by MTT assay. RESULT: The vital activity of PBMCs in the SM-exposed group with the mitogen was significantly lower than that in the control group (P = 0.016). Whereas, there was no significant difference in the viability of PBMCs without the mitogen between two groups. Furthermore, hematologic findings indicated that the SM-exposed group had a significant increase in the total count of WBC, neutrophil, MCV, and HCT values but the lymphocyte count and MCHC value were significantly lower than those in the control group. CONCLUSION: Exposure to SM even after a long time, can affect hematologic parameters and vital activity of PBMCs.