Antibacterial activity of Curcuma longa ethanolic extract against Acinetobacter baumanii isolated from burn wound infection and molecular typing of them.

Mohammad Mehdi Attarpour Yazdi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137408
Date :  2019/04/18
Publish in :    The 12th International & 17th National Congress on Quality Improvement in Clinical Laboratory


Keywords :Acinetobacter baumanii, Antibacterial activity, Curcuma longa, Burn wound infection, Molecular typing

Abstract :

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Introduction & Aims: Acinetobacter baumanii are important pathogen to produce burn wound infections. Increasing of antibiotic usage for their infections, created antibiotic resistance. Medical herbs have anti-microbial activity. The aims of this study was to determine the antibacterial activity of ethanolic extract of Curcuma amada rhizome against Acinetobacter baumanii isolated from burn wound infections and to compare with effects of selected antibiotics and molecular typing of them In- vitro. Methods: First, a sample of ethanolic extract of the plant rhizome was prepared by maceration method. Then its antibacterial activity against 93 isolates of Acinetobacter baumanii from 300 samples of different infection was evaluated by well diffusion and agar serial dilution method. All the resistant isolates were the subjected to PCR assay for bla CTX-M, bla TEM and bla SHV genes that encode ESBL. Results: The results demonstrated that the Curcuma amada rhizome ethanolic extract had been affected against 56 of Acinetobacter baumanii isolated (60.2).The MIC of the extract was 15.50 mg/ml. There was significant difference between the effects of plant and antibiotics on Acinetobacter (P0.05). Among 37 Acinetobacter baumanii isolates that were resistance to Curcuma amada rhizome ethanolic extract, 32 and 4 isolates were CTX-M and TEM producer, respectively. bla – SHV was not detected in any of isolates. Discussion & Conclusions: This study demonstrates that an ethanolic extract of Curcuma amada is effective on Acinetobacter baumanii isolated from burn wound infection and its effect is even better than that of selective antibiotics. Further investigations will be necessary.