Antibacterial activity of Curcuma longa ethanolic extract against Pseudomonas aeruginosa isolated from burn wound infection and molecular typing of them

Mohammad Mehdi Attarpour Yazdi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137409
Date :  2019/04/21
Publish in :    The 12th International & 17th National Congress on Quality Improvement in Clinical Laboratory


Keywords :Pseudomonas aeruginosa, Antibacterial activity, Curcuma longa, Burn wound infection, Molecular typing

Abstract :

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Introduction & Aims: Pseudomonas aeruginosa is important pathogen to produce burn wound infections. Increasing of antibiotic usage for their infections, created antibiotic resistance. Medical herbs have anti-microbial activity .The aims of this study was to determine the antibacterial activity of ethanolic extract of Curcuma amada rhizome against Pseudomonas aeruginosa isolated from burn wound infections and to compare with effects of selected antibiotics and molecular typing of them In- vitro. Methods: First, a sample of ethanolic extract of the plant rhizome was prepared by maceration method. Then its antibacterial activity against 122 isolates of Pseudomonas aeruginosa from 300 samples of different infection was evaluated by well diffusion and agar serial dilution method. All the resistant isolates were the subjected to PCR assay for bla CTX-M, bla TEM and bla SHV genes that encode ESBL. Results: The results demonstrated that the Curcuma amada rhizome ethanolic extract had been affected against 94 of Pseudomonas aeruginosa isolated (77).The MIC of the extract for Pseudomonas was14 mg/ml. There was significant difference between the effects of plant and antibiotics on Pseudomonas aeruginosa (P0.05). Among 100 Pseudomonas aeruginosa isolates that were resistance to Curcuma amada rhizome ethanolic extract, 50 and 20 isolates were CTX-M and TEM producer, respectively. bla – SHV was not detected in any of isolates. Discussion & Conclusions: This study demonstrates that an ethanolic extract of Curcuma amada is effective on Pseudomonas aeruginosa isolated from burn wound infection and its effect is even better than that of selective antibiotics. Further investigations will be necessary.