The effect of nobiletin on behavioral and histological alterations in a model of Parkinson’s disease induced by intranigral injection of lipopolysaccharide in the rat

Maryam Khorasani | Marzieh Fakour | Reihane Ghasemi Tarei | Sedigheh Keshtkar | Zahra Kiasalari Reineh | Mehrdad Roghani

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137483
Date :  2019/10/30
Publish in :    24 امين کنگره ملي و سومين کنگره بين المللي فيزيولوژي و فارماکولوژي ايران


Keywords :Parkinson Disease, Nobiletin, Behavioral changes, Lipopolysaccharide, Dopaminergic

Abstract :

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Background and Objective: Anti-inflammatory property of nobiletin, a compound in citrus fruits peel, has been shown. Besides, neuroinflammation is involved in triggering and progression of neurodegenerative disorders such as Parkinson’s Disease (PD). The purpose of this study was to determine the effect of nobiletin on behavioral and histological changes in an experimental model of PD induced by intranigral injection of lipopolysaccharide (LPS) in rats. Materials and Methods: In this study, to generate PD model, 5μg of LPS was injected into right midbrain of rats through stereotaxic surgery. Nobiletin was administrated daily for one week after the surgery at a dose of 10 mg/kg p.o. via gavage. Behavioral changes were evaluated by Y maze, Elevated plus maze, Passive avoidance, Novel object recognition, Forced swimming, and Rotational tests. For histological assessments, number of dopaminergic neurons was counted. Results: Findings of this study demonstrated that administration of nobiletin to parkinsonian rats induced by LPS resulted in a significant decrease in the number of rotations and immobility time, and an insignificant decrease in percentage of alternation and significant increase of novel object discrimination and insignificant increase in the percentage of open arm spending time. Also, nobiletin treatment prevented loss of dopaminergic neurons. Conclusion: Nobiletin treatment alleviated motor asymmetry and caused improvement of learning and memory and depressive like disorder in PD model induced by LPS and part of its beneficial effect is mediated via its neuroprotective effect.