Shahed University

The effect of nobiletin on inflammatory response, oxidative stress, cholinesterase, and apoptosis in amyloid beta-induced model of Alzheimer’s disease in the rat

Reihane Ghasemi Tarei | Zahra Kiasalari Reineh | Marzieh Fakour         | Maryam Khorasani | Sedigheh Keshtkar | Mehrdad Roghani

URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137487
Date :  2019/10/30
Publish in :    24 امين کنگره ملي و سومين کنگره بين المللي فيزيولوژي و فارماکولوژي ايران


Keywords :Alzheimer’s disease, Nobiletin, Oxidative stress, Inflammation, Cholinesterase, Apoptosis

Abstract :
Background and Objective: Alzheimer’s disease (AD) is the most common cause of dementia. Nobiletin as a natural compound from citrus peels possesses anti-dementia activity. In addition, nobiletin could ameliorate oxidative stress and inflammation. Therefore, this study was conducted to assess the effect of nobiletin on hippocampal inflammatory response, oxidative stress, cholinesterase, and apoptosis in amyloid beta-induced model of AD in the rat. Materials and Methods: In this study, 32 male Wistar rats were randomly divided into four groups, including: 1. sham, 2. sham+nobiletin, 3. amyloid beta, 4. amyloid beta+ nobiletin. Rats were injected bilaterally with amyloid beta into the CA1 region of the hippocampus through stereotaxic surgery. Nobiletin was administered at a dose of 10 mg/kg daily one hour after surgery for one week via gavage. Then, oxidative stress markers (MDA, ROS, GSH, SOD, Nrf2, catalase), inflammatory factors (NF-κB, TNF, TLR4), apoptosis parameter (DNA fragmentation) and AChE activity were measured in hippocampal homogenate. Results: Treatment of nobiletin significantly reduced MDA, ROS and increased SOD with no significant change of GSH, catalase and Nrf2. Another beneficial effect of nobiletin was reduction of inflammatory factors. Besides, nobiletin did not significantly change AChE activity and DNA fragmentation. Conclusion: According to the findings of this study, we can say that treatment with nobiletin reduces inflammation and oxidative stress. However anti-AChE and anti-apoptotic effects of this agent need to be evaluated more.


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