Comparison of Routine and Microwave-assisted Decalcification of Bone with or without Teeth, a Histologic Study

Niloofar Hajihoseini | Shahroo Etemad Moghadam | Gita Rezvani

---

URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137823
Date :  2020/09/24
Publish in :    پنجمين همايش اسيب شناسان دهان، فک و صورت ايران


Keywords : Bone and Bones Decalcification Technique; Microwaves; Tooth

Abstract :

---

Background: The aim of this study was to compare conventional and microwave-assisted decalcification of sheep bone with and without teeth, to detect any differences in tissue-detailpreservation, staining-quality and rate of decalcification. Materials and Methods: Twenty-four specimens consisting of 12 blocks of mandibular molars with their surrounding bone and 12 blocks of mandibular osseous-tissue were allocated into two microwave- or routine-decalcification groups using 5 nitric- or formic-acid as decalcifying agents. In addition to decalcification rate, a number of variables were used to assess staining quality and tissue-detail-preservation which were compared between the two groups using ManWhitney test (P0.05). Results: Time to complete decalcification was significantly reduced in the microwave-treated samples as compared to the conventional method, regardless of the decalcifying agent (P=0.025). For both acids, most variables related to staining quality and tissue-detail-preservation were similar between the techniques (P0.05). However, in general the performance of formic acid was slightly inferior to nitric acid, especially for specimens containing both tooth and bone. Conclusion: The use of microwave technology can accelerate decalcification of bone and teeth of sheep mandibles and at the same time preserve tissue structure and staining quality at an acceptable level. Further studies are required to help select the superior acid, especially in specimens containing bone and teeth.