Intra-hippocampal L-NAME low dose did not affect morphine antinociception in the rat formalin test

Manizheh Karami | Mohammadreza Zarrindast | Hashemi

---

URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137889
Date :  2019/10/30
Publish in :    بيست و چهارمين کنگره فيزيولوژي و فارماکولوژِي ايران

Link :  http://cong24.phypha.ir/
Keywords :Key Words: Morphine, L-NAME, NO, Antinociception, Formalin test

Abstract :

---

NG-Nitro-L-arginine Methyl Ester (L-NAME), the NO synthase inhibitor, intra-CA1 injection to interfere with the morphine anti-pain effect using rat formalin test. Methods: Male Wistar rats in group control received subcutaneous (s.c.) injections of formalin (50 μl at 2.5) once prior to testing. Morphine identified dose (6 mg/kg) was injected intraperituneally (i.p.) 10 min before injection of formalin. L-NAME (0.25-2 μg/rat) pre-administered (5 min) prior to the morphine injection pre-testing. Early and late phases of the formalin test were calculated and compared with the morphine as well as the control data. Results: Morphine in comparison to the control induced a significant anti-pain response both in early and late phases; however, L-NAME low (0.25 μg/rat) rather higher doses did not reverse that response. Conclusion: L-NAME low dose, based on the finding, most probably cannot interact with the modulator NO, so, it did not affect the antinociception induced of morphine in the rat hippocampal CA1.

http://cong24.phypha.ir/