Shahed University

Indicated and non-indicated antibiotic administration during pregnancy and its effect on pregnancy outcomes: Role of inflammation

Amir Hossein Norooznezhad | Shima Javidan | Mohammad Hossein Ahmadi | Tooba Ghazanfari | Leila Norouz | Azadeh Farzanehdoust | Kamran Mansouri | Shayan Mostafaei | Pouya Javadian | Mahdi Sheikh | Sedigheh Hantoushzadeh

Date :  2020/12/01
Publish in :    International Immunopharmacology

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Keywords :Antibiotic Preterm labor, Inflammation, Interleukin 1 ?, Interleukin 6, Tumor necrosis factor ?

Abstract :
The objective of this study was to compare the release of endotoxin and pro-inflammatory cytokines as well as pregnancy outcomes after antibiotic exposure in healthy and bacterial infected pregnant rats. Thirty female Wistar pregnant rats were divided into five groups. Group A considered as control and received intraperitoneal saline 0.9 on 17th day of gestation or DG) and groups B and C treated with 20 mg/kg/day intravenous ceftriaxone and ceftazidime, respectively (DG: 18–20). Groups D and E received intraperitoneal E. coli and LPS on 17th DG respectively. Also, groups F and G received the same treatment as group D but they treated with the exact antibiotics mentioned for groups B and C (same dose and duration). Pregnancy outcomes as well as maternal sera levels of endotoxin, tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and IL-6 were assessed using enzyme-linked immunosorbent assay. It was shown that group B had a higher IL-1β (P = 0.003) and TNF-α (P = 0.003) levels compared to the controls (CTC). Group C expressed a lower gestational duration (P = 0.007) as well as higher IL-6 (P = 0.025) and TNF-α (P 0.001) levels CTC. Interestingly, both group B (P = 0.021) and C (P 0.001) had a higher rate of endotoxin release CTC. Moreover, in group C, IL-6 (P 0.0001 and r = −0.941) had a significant correlation with gestational duration. As the results showed, antibiotic administration in non-indication condition seems to be associated with significantly higher production of endotoxin and inflammatory cytokines which increase the risk of poor pregnancy outcomes.