Shahed University

Alcea aucheri: evaluation of its anticonvulsant effect in pentylenetetrazole and maximal electroshock seizures in mice

Tajmah Mombeini | Babak Asadpour Behzadi | Ramtin Ejtemaei | Freidoun Tahmasbi

Date :  2020/12/09
Publish in :    9th Basic & Clinical Neuroscience Congress 2020

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Keywords :Alcea aucheri, Pentylenetetrazole, Maximal electroshock, Seizure

Abstract :
We have showed previously that both of single and repeated dose treatment with the aqueous extract of Alcea aucheri (EFA) has anxiolytic and, a dose-dependent sedative effects in rats. Our preliminary phytochemical analysis showed the presence of phenolic compounds, polysaccharides, and flavonoids in the extract. The flavonoids have a selective affinity for central benzodiazepine receptors and some of them possess a pharmacological profile compatible with a partial agonist action. In addition, GABAA ergic drugs are the mainstay of treatments to suppress seizures. Therefore, this study was designed to investigate anticonvulsant effect of aqueous extract of flowers of Alcea aucheri (EFA)in mice. Methods: Seizures were induced in male adult mice by administration of Pentylenetetrazol (PTZ) or Maximal Electroshock (MES). Mice were randomly subjected to receive saline, EFA (8.75-175, or diazepam intraperitoneally (i.p.) 15 or 30 min before intravenous PTZ injection (i.e. PTZ-15, PTZ-30). In another experiment, mice were treated (i.p.) with saline, EFA (8.75-350, or phenytoin 15 or 30 min before the MES test (i.e. MES-15, MES-30). Diazepam and phenytoin were used as positive control drugs. Results: Our findings showed that EFA increased the PTZ seizure threshold in the PTZ-15 test. In the MES test, EFA increased the latency to onset of seizure at both time points, decreased seizure duration, and protected mice against seizure in the MES-30 test. Furthermore, EFA at all doses reduced the mortality rate of mice after electroshock convulsion. Conclusion: These findings suggest that Alcea aucheri possibly have anticonvulsant effects in PTZ and MES-induced seizure models in mice. The phenolic acids, polysaccharides and/or flavonoids may be contributed to the observed effects.

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