Changes in fatty acid composition in spinal cord of ethidium bromide-treated rats as a multiple sclerosis (MS) model; GC-MS evaluation

Nafise sadat Mirshafian | Majid Hassanpour-Ezatti | Hossein Dehghan

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=158883
Date :  2019/10/09
Publish in :    بيست و چهارمين کنگره فيزيولوژي و فارماکولوژِي ايران

Link :  https://civilica.com/l/10100/
Keywords :Fatty acid, Gas Chromatography-Mass Spectrometry, Ethidium bromide, Multiple Sclerosis, Spinal cord, Rats

Abstract :

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The compositions of fatty acids (FAs) in nervous tissues of animal models of MS may have important roles in the process and mechanisms related to the onset and progress of this disease. The purpose of this study was to evaluate the effects of ethidium bromide (ET), which used for induction of neurodegenerative symptoms in rats, on spinal FAs profile in rats. The present study used gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid methyl esters (FAMES) to profile all detectable FA species present in rats spinal cord tissue homogenate after treatment with intraspinally injection of ET (0.02mg/ml). Quantitative analysis was done on spinal cord samples of normal and ET-treated rats (n=6 per group). GC-MS analysis of FAMES detected a total of 24 FAs and of these, 13 were fully quantifiable. The results showed significant alterations in ET-treated rat spinal cord FA concentrations. A total of 3 FAs were elevated in ET-treated samples with arachidic, oleic and eicosenoic acid increased most (100; P=0.033). Docosahexanoic acid, Vaccenic acid and linoleic acid concentrations were decreased (47; P=0.018) which accordance with the findings of others in other region of nervous after the onset of MS. Furthermore, our results appear to indicate that alteration of FAs at spinal level in ET model of MS in rats. These preliminary findings pinpoint FAs disturbances at spinal cord level have been occurred and may be potentially important in the pathology of MS.

https://civilica.com/l/10100/