Marham-Mafasel decrease joint inflammation and IL-1? gene expression in rheumatoid arthritis animal model

Roya Yaraee Shahmirzady | Mohammad Majidi | Fatemeh Heidarnejad | Mohsen Naseri | Shahin Bonakdar | Maryam Salimi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=158936
Date :  2021/05/03
Publish in :    Veterinary Medicine and Science
DOI :  https://doi.org/10.1002/vms3.430
Link :  http://dx.doi.org/10.1002/vms3.430
Keywords :adjuvant, cytokine, herbal medicines, joint inflammation

Abstract :

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Background: Rheumatoid arthritis (RA) is a systemic chronic disease with synovial membrane, tendon and articular tissue inflammation. Current treatments of RA have many side effects and are quite expensive. Today, new treatments procedures and inexpensive herbal drugs are developed. Marham-Mafasel is mainly made out of two traditional herbs (Arnebia euchroma and Martricaria chamomilla). Objective: In this study, for the first time, the impact of Marham-Mafasel on joint inflammation, histopathological changes and IL-1β gene expression was evaluated in RA animal model. Methods: The RA was induced by a single s.c. injection of 0.1 ml Freunds complete adjuvant into the left hind footpad. In continuous, 15 RA male Wistar rats were used in three groups: I: Control; II: Treatment I (Piroxicam) and III: Treatment II (Marham-Mafasel). The volume of the hind paw was measured every day from 0 to 19 using water changed volume approach. The inflammation in the joint was evaluated using histopathology assay and gene expression of IL-1β was evaluated with use of Real-Time PCR. Results: Hind paw swelling of Marham-Mafasel at days 10th and 19th was reduced compared with the control group (p 0.05). There was no statistically difference in histological degrading and changes index in three groups (p ≥ 0.05). Relative expression of IL-1β in Marham-Mafasel group was significantly decreased compared with other groups. Conclusion: The co-administration of M. Chamomile and A. euchroma, called Marham-Mafasel, decreases IL-1β gene expression that leads to a reduction in inflammation in rheumatoid arthritis (RA) animal model.