Therapeutic and preventive effects of exercise training on metabolic regulators/markers in mouse colorectal cancer cells

Amin Isanejad | Hossein Shirvani | Behzad Bazgir | Mostafa Rahimi | Mohammad Samadi | Vahid Sobhani | Ali Mohammad Alizadeh | Ehsan Arabzadeh

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=159258
Date :  2021/12/01
Publish in :    Sport Sciences for Health
DOI :  https://doi.org/10.1007/s11332-021-00826-8
Link :  http://dx.doi.org/10.1007/s11332-021-00826-8
Keywords :GLUT1, MICT1 and PFK1

Abstract :

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Background The enzymes and metabolic controllers of tumor cells are affecting by the regulation of physical activity and exercise training. Aim The aim of this study was to investigate the role of preventive and treatment of aerobic interval training in colon cancer with determining the protein values of MCT1, GLUT1, PFK-1 and expression of the p53 gene in tumor cells in the animal model. Methods Forty-eight male BALB/c mice (6–8 weeks old) participate in this study. Animals were equally randomized into 6 groups: I: control (C); healthy animals, II: Exercise (E), III: tumor induction (T); animals received AOM for inducing colon cancer, IV: AOM + exercise (TE); animals with colon cancer underwent 8 weeks of the exercise training protocol after tumor establishment, V: exercise + AOM (ET); animals received exercise protocol 1 week before AOM consumption, and (VI) exercise + AOM + exercise (ETE); animals received exercise protocol one week before and after AOM consumption (about 15 weeks) Groups III–VI were weekly-received AOM (as carcinogenic agent, 10 mg/kg s.c) in three consecutive weeks to induce colon cancer. Protein levels of GLUT1, MICT1 and PFK1 in solid colon tumor was measured by ELISA method. Also we used RTPcr for gene expression of P53. GAPDH were used as reference genes. Results The results showed that the tumor significantly increased MCT1 in the tumor group compared to the control group (p = 0.001), Also, exercise before and after tumor induction reduced MCT1 in the colon (respectively: p = 0.02 and p = 0.01). Levels of GLUT1 colon in the tumor group showed a significant increase compared to the control group (p = 0.001), that exercise after tumor induction was effective in reducing this protein (p = 0.01). PFK-1 had a significant decrease in ET and TE groups compare to T group (p = 0.02 and p = 0.01, respectively). While the p53 gene decreased significantly in the tumor group compared to the control group (p = 0.01) Exercise before tumor induction and after tumor induction increased significantly (p = 0.01 and p = 0.04, respectively) in this gene compared to the tumor group. Conclusion Long-term aerobic training can act as a preventive or inhibitor of tumor progression by acting as an adjunct in the treatment of colon cancer. It seems that this preventive and treatment effects exercise training can attribute to regulation of lactate and glucose transporters by up-regulation of p53 colorectal cancer cell.