Fumigant Toxicity of the Essential oil of Saatar Zataria multiflora on tomato leaf miner, Tuta absoluta (Lepidoptea: Gelechiidae

Samira Gooderzvand | Habib Abbasipour | Jabber Karimi | Alireza Askarianzadeh

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=42253
Date :  2016/08/27
Publish in :    بيست و دومين کنگره گياهپزشکي ايران

Link :  https://ippc22.ut.ac.ir/
Keywords :Fumigant, Zataria, multiflora, tomato, miner, absoluta, Gelechiidae

Abstract :

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The tomato leaf miner (TLM), Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is an important pest on tomato, potato and other Solanaceous with a great economic importance. One of the main tools in its management, is the use of conventional synthetic insecticides, however this overreliance on the use of synthetic insecticides quickly leads to problems of insecticide resistance. The use of natural compounds such as plant essential oils are considered as a alternatives to chemical pesticides due to their lower toxicity on the non-target and low persistence in the environment. In this research, fumigant toxicity Zataria multiflora essential oil was studied on egg, the 2nd larval instars and adults. The essential oil was obtained by hydrodistillation method, using a modified Clevenger-type apparatus. Experiments were carried out at 27 ± 2°C and 65 ± 5 R.H. The fumigant toxicity of essential oil on 2nd larvae (inside leaf) and egg were tested in macro plastic container volume 1800 ml, The vials were contained leaves containing larvae mines with ten larvae (2nd instars) or 20 eggs, separately and For 2nd larvae (outside leaf) and adults bioassay experiments in glass vial volume 600 ml that contained ten larvae (2nd instars) or 10 adults, separately. No. 1 Whatman filter paper disks attached to the undersurface of vials. Filter papers were impregnated with series of pure concentrations of essential oil ranging from 20-640 μl L-1 air, 2-10 μl L-1 air, 0.8-2.5μl L-1 air and 1-2 μl L-1 air were used in the main bioassay tests on egg, the 2nd larval instars (in mines, directly) and adults, respectively and each concentration and control included three replicates. The mortality was recorded after 48 hours and LC50 values for egg, 2nd larvae (in mines, directly) and adults were calculated. Based on Probit analysis, the LC50 values for egg, 2nd larvae (in mines, directly) and adults were 60.26, 4.44, 1.26 and 1.37 μl L-1 air, respectively. The type and amount of constituents of the essential oils were analyzed by GC/MS. The major constituents of Z. multiflora were Thymol (33.52), Carvacrol (14.37) and γ.- terpinene (8.50). Our results showed that adult stage and second instar larvae, outside leaf of T. absoluta was more sensitive to Z. multiflora essential oil than egg and second instar larvae, inside leaf of this pest.

https://ippc22.ut.ac.ir/