Different expression of RbohD (respiratory burst oxidase - D ) gene and H 2 O 2 content between contrasting Festuca arundinaceae accessions with respect to drought resistance under water deficient condition

Iman Rohollahi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=42377
Date :  2016/09/10
Publish in :    The challenge to produce more food and energy with sustainability


Keywords :burst, content, contrasting, Festuca, accessions, respect, drought, resistance, water, condition

Abstract :

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The objectives of this study were to investigate sequencing and expression level of FrbohD gene in leaf and shoot under drought, after establishment upon selected entry in germination stage for Festuca arundinacea. F. arundinaceae is a major cool-season forage and turf grass specie which is adapted to cold, arid and semiarid environments. The populations were evaluated under 100 field soil water capacity (FC) -0.33 matric potential (MPa) and at 80, 60 and 40 FC. Isfahan had the best final emergence, longer leaf and root length compared to Barvado at 40 FC. We selected three entries on the emergence and early establishment of 14 wild F. arundinacea populations collected from various regions in Iran, and two commercial turf cultivars. After sequencing of FrbohD, expression level of FrbohD gene response to drought in a drought-tolerance entry (Isfahan) and a drought-sensitive entry (Quchan) were compared to those of cultivar Barvado. The plants were subjected to 4, 6 and 8-day without water before samples were taken from the leaf and shoot for the purpose of gene expression investigation. Henceforth, consistent with germination results, the transcript level of FrbohD substantially increased under drought stresses in the leaf and shoot of Quchan entry compared to cultivar Barvado as reference gene. Significantly, lower expression FrbohD gene in Isfahan entry might be as a result of the higher antioxidant enzyme activity and/or low ROS accumulation in Isfahan entry compared to those in Quchan entry. Our results showed that up-regulated of FrbohD gene in leaf under drought in Quchan and Isfahan entries compared to their control. This higher level of FrbohD gene transcript in a leaf following drought stress compared to control, indicating that FrbohD gene could be involved to mediate drought stress effect.