Shahed University

EFFECT OF INTRA-CENTRAL AMYGDALA SILVER NANOPARTICLES ON EXPRESSION OF NALOXONE- INDUCED WITHDRAWAL BEHAVIORAL SIGNS

Mahnaz Rahimpour | Manizheh Karami | Ali Haeri Rohani

URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=85461
Date :  2018/09/05
Publish in :     Annual International Addiction Science congress 12

Link :  http://ascongress.ir
Keywords :morphine, naloxone, silver nanoparticles, withdrawal, behavioral signs

Abstract :
Background and Aim : Withdraw to dependence on morphine may cause conditioned place aversion (CPA). It may serve as a highly sensitive measure reflective of the negative motivational aspect of opiate withdrawal in animal model. Silver Nanoparticles (SNPs) owing to their small size and large ratio of surface area to volume, can interact with biomolecules and penetrate cell and nuclear membranes and induce effects. The purpose of this research was to study the effect of SNPs on naloxone induced withdrawal behavioral signs in morphine conditioned rats. Methods : Male Wistar rats (300-350 g) were cannulated bilaterally by Stereotaxic apparatus for the CeA (Anteriorposterior= –2.12 mm posterior to bregma and lateral= ±4.1 mm; dorsoventral=7.8 mm, according to the atlas of Paxinos and Watson (Paxinos and Watson, 1997) coordinates. Morphine (2.5-7.5 mg/kg) was injected s.c. once a day in the conditioned place preference (CPP) box. On test day, the administration of naloxone (0.05- 0.4 µg/rat) was intra-CeA 5 min before testing. SNPs (0.0001- 0.01 µg/rat) were intra-CeA injected 10 min before microinjection of naloxone. Data in all groups were compared by ANOVA (one- and/or two-way analysis of variance). Results : Though microinjection of naloxone into CeA (0.4 µg/rat) did not cause meaningful withdrawal response in the morphine CPP model, however, a microinjection of silver nanoparticles (0.001, 0.0001 µg/rat) prior to administration of naloxone had significant effect on expression of withdrawal symptoms. Conclusion : SNPs may change in sign of withdrawal induced of naloxone in CPP animal model.

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