Comparison of Hematoxylin-eosin, Reticulin and Periodic acid–Schiff stains in demonstrating the basement membrane pattern of Oral Squamous Cell Carcinoma

Noushin Jalayer Naderi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=105888
Date :  2018/12/05
Publish in :    همايش ساليانه اسيب شناسي ايران


Keywords : Carcinoma, squamous cell, Basement membranes, Oral cavity, Stain

Abstract :

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Comparison of Hematoxylin -eosin, Reticulin and Periodic acid–Schiff stains in demonstrating the basement membrane pattern of Oral Squamous Cell Carcinoma Noushin Jalayer Naderi 1* 1. Oral and Maxillofacial Dept., Faculty of Dentistry, Shahed University jalayer@shahed.ac.ir Abstracts Introduction and Aim: Thickness and pattern changes in malignant epithelial tumors are indicating signs of invasion. Diagnosing the basement membrane changes is a useful tool to determine the invasion especially in small samples. The aim was to compare the Hematoxylin -eosin, Reticulin and Periodic acid –Schiff (PAS) staining in demonstrating the basement membrane changes of Oral Squamous Cell Carcinoma (OSCC). Materials and Methods: The study was descriptive, nonparametric. 3μ sections of fixed paraffin embedded tissue of 11 OSCC and 5 normal mucosa samples were stained with Hematoxylin -eosin, Reticulin and Periodic acid –Schiff .The thickness and pattern of basement membrane were determined under different stains and compared wi th normal mucosa. Results: In sectioned obtained with different staining, the basement membrane of normal epithelium was extended and constant with 2 μ thickness. The basement membrane in invasive parts of OSCC was indistinct and discontinuous with Hematox ylin -eosin staining. PAS was more precise in demonstrating the basement membrane thickness and pattern. In compare to Hematoxylin- eosin and PAS, the Reticulin stain demonstrated the basement membrane thickness and pattern more precisely. Conclusion: Reticu lin in comparing to Hematoxylin -eosin and PAS was more practical stain in demonstrating the basement membrane changes of OSCC. Keywords Carcinoma, squamous cell, Basement membranes, Oral cavity, Stain