Evaluation of Dionaea muscipula vitreous plant growth through tissue culture

Iman Rohollahi | Ayatollah Rezaei | Mona Noormohammadi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=105986
Date :  2018/10/25
Publish in :    دومين کنگره بين المللي و سومين کنگره ملي گل و گياهان زينتي ايران

Link :  http://isopcongress.ir/fa/
Keywords :Venus flytrap, Tissue culture, Micropropagation, Cytokinin

Abstract :

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Tissue culture is one of the most effective methods for plants mass production, especially for greenhouse crops. The most important features of plant propagation in this way are to preserve the traits of the mother plant, mass production and prevent the transmission of viral diseases. For this purpose, venous flytrap plantlets were examined as explants in MS medium containing TDZ, Kin, BA and 2iP hormones in 4 levels (5, 10, 15 and 20 μM). After 90 days, traits such as root length, number of roots, leaf length, leaf number, survival rate of seedlings after adaptation and number of seedlings in each treatment, and then the amount of phenol, flavonoid, anthocyanin and amount of pigment photosynthesis were evaluated. The results showed that most of the traits were significantly affected by hormones. Physiological traits including phenolic compounds, flavonoids, anthocyanins and photosynthetic pigments increased with increasing hormones concentration. Most morphological traits also increased with increasing hormonal concentrations. The highest mean values were observed in TDZ hormone treatment and the growth parameters and photosynthetic pigments content (chlorophyll a and b and carotenoids) and the content of anthocyanins, phenols and flavonoids in this treatment increased significantly. Maximum traits were obtained in 20 μM hormone TDZ treatment. The treatment with TDZ hormone at the highest concentration had the greatest effect on growth improvement and leaf number, leaf length, leaf length, root length, root number per implant and physiological traits such as photosynthetic pigments (chlorophyll a and b and carotenoids) and the content of anthocyanins, flavonoids and total phenolics. Treatment with hormone 2ip had the least effect on these traits.

http://isopcongress.ir/fa/