Assessment of the Immunomodulatory Effects of Some Allium species on TNF-? and NO Production

Hosseinpur Z | Tayebeh Radjabian | Tooba Ghazanfari | Nasiri Z

---

URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=106103
Date :  2018/04/28
Publish in :    14th Internatinal Congress of Immunology

Link :  http://icia.ir/
Keywords :Allium species, Bulb extract, Macrophage, NO, TNF-?

Abstract :

---

Background: The effects of Allium sativum (garlic) extract, as a medicinal plant, have been demonstrated on variety of immune cells and their secretory mediators associated with various immune-related diseases. According to the efficiency of garlic bulb extracts in animal studies and unveiled data about the other Allium species on immune responder cells, the effects of aqueous extracts from the bulbs of some wild grown Allium species from Iran on TNF-α and NO generation in BALB/c mice macrophages were evaluated. Methods: The bulbs of some Allium species were collected from their natural localities and the aqueous extracts of them were prepared. Peritoneal macrophages were isolated from BALB/c mice and were incubated with different concentrations of the bulb extracts. Then, the amounts of TNF-α, and NO production in macrophages were measured. Results: In non-pathogenic conditions of this research, NO generation in macrophages was increased after incubation with bulb extracts of A. elburzense and A. Asarense, but their stimulatory effects was not significant. The effects of different concentrations of the bulb extracts of the examined Allium species were not significant on TNF-α generation, except for 0.05 mg/ml (P0.001) bulb extract of A. sativum. In spite of the fact that the immunomodulatory effects of the bulb extracts on TNF-α and NO production were not significant, but the identical patterns were observed for the stimulatory effects of the bulb extracts of A. elburzense and A. Asarense on the production of these factors in the pretreated macrophages. Also, similar patterns were obtained in reduction of NO production by macrophages after treatment with the bulb extracts of A. sativum and A. Iranicum. Conclusion: In general, bulb extracts of the Allium species had modulatory effects on the studied mediator factors, which are involved in immune related-diseases, and these species may be considered for treatment studies.

http://icia.ir/