Evaluation of Allium asarense and Allium sativum Aqueous Extracts on Macrophages

Hatami H | Hosseinpur Z | Tayebeh Radjabian | Ghazanfari T

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=106105
Date :  2018/04/28
Publish in :    14th Internatinal Congress of Immunology

Link :  http://icia.ir/
Keywords :Allium sativum, Allium asarense, Nitric Oxide, TNF-?

Abstract :

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Background: It has been showed that some of Allium species have immunomodulatory effects on human immune system. Recent studies on A. cepa and A. sativum showed that their extract have therapeutic effects including anti-cancer, anti-diabetes, anti-oxidant activities. In this research we performed a comparative study about the effects of bulb extracts from wild A. asarense and cultivated A. sativum on macrophages responses. Methods: Peritoneal macrophages were isolated from BALB/c mice and were incubated with various concentrations of the aqueous bulb extracts of the studied Allium species. Then, the amounts of TNF-α and NO generation and MTT in macrophages were measured. Results: Our results showed that the bulb extracts of both Allium species had positive effects on macrophages viability, but the significant stimulatory effects of A. asarense bulb extract were observed at the lower concentrations (≤0.01 mg/ml). Also significant increases were found in TNF-α concentration after treatment with 0.1, 0.05 and 0.001 mg/ml of the A. asarense bulb extract and 0.05 mg/ml A. sativum bulb extract. No significant changes were observed in nitric oxide levels after treatment with the extracts. Conclusion: Generally, it seems that in addition to A. sativum, A. asarense could be used as an effective medicinal plant for immunoregulation.

http://icia.ir/