Isolation and expression analysis of the RbohD gene (gene encoding respiratory burst oxidase) in Festuca arundinaceae cv. Barvado and two selected accessions under drought stress

Iman Rohollahi | Mohsen Kafi | nayer azam khoshkholgh sima | Alireza Khaleghi | Toshi Hiko Yamada

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=10730
Date :  2015/05/24
Publish in :    اولين همايش بين المللي و نهمين همايش ملي بيوتکنولوژي جمهوري اسلامي ايران

Link :  http://http://biotechcongress.ir
Keywords :Isolation, encoding, burst, Festuca, accessions, drought, stress

Abstract :

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The objectives of this study were to investigate sequencing and expression level of FrbohD gene and H2O2 content in leaf and shoot under drought, after establishment upon selected entry in germination stage for Festuca arundinacea. F. arundinaceae is a major cool-season turf grass specie which is adapted to cold, arid and semiarid environments. The entries were evaluated under 100 field soil water capacity -0.33 matric potential (MPa) and at 80, 60 and 40 field capacity (FC). Isfahan had the best final emergence, longer leaf and root length compared to Barvado at 40 FC. We selected three entries on the emergence and early establishment of 14 wild F. arundinacea entries collected from various regions in Iran, and two commercial turf cultivars. After sequencing of FrbohD in Barvado, expression level of FrbohD gene response to drought in a drought-tolerance entry (Isfahan), drought-sensitive entry (Quchan) and cultivar Barvado were compared to their controls respectively. The plants were subjected to 3, 6 and 9-day without water before samples were taken from the leaf and shoot for the purpose of gene expression investigate and endogenous amount of H2O2 quantification. Henceforth, consistent with germination results, the transcript level of FrbohD and H2O2 content substantially increased under drought stresses in the leaf and shoot of Quchan entry (drought sensitive) compared to control than those in cultivar Barvado. Also, lower FrbohD gene expression and H2O2 content in Isfahan entry (drought resistance) under drought might be as a result of the higher antioxidant enzyme activity compared to control. Higher level of FrbohD gene transcript and H2O2 concentration level in a leaf following drought stress compared to control, indicating that FrbohD gene could be involved to mediate drought stress effect. Keywords: Emergence, Drought tolerance, Sequence, H2O2.

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