GERMINATION INDICES AND ANTIOXIDANT ACTIVITY ENZYME RESPONSES OF MADAGASCAR PERIWINKLE (CATHARANTHUS ROSEUS (L.) G. DON) UNDER PRETREATMENT BY SALICYLIC ACID

M Ababaf | H Omidi | A M Bakhshandeh

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=116457
Date :  2019/04/19
Publish in :    Applied Ecology and Environmental Research
DOI :  https://doi.org/10.15666/aeer/1702_39894005

Keywords :ACID

Abstract :

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The present factorial experiment was conducted based on completely randomized design (CRD) with three replications in order to determine the optimum time and concentration for Catharanthus roseus seed priming with salicylic acid. Treatments included five different levels of salicylic acid (0; 0.01; 0.1; 0.5 and 1 mM) as well as five different periods of time (0, 6, 12, 24, and 48 hours). Results indicated that the effects of concentration and time of priming were significant on all the studied traits. In addition, the effect of the interaction between concentration and priming time was significant on the mean time of germination, seedling length vigor index, chlorophyll content, and the activity of antioxidant enzymes. Further, the germination percentage increased by 24.1 and 22.3 in the treatment of 1 mM, and 48 hours, respectively, compared to the control. The highest germination speed was in 1 mM and 48-hour treatment, which had a 40 higher increase than the control. The interaction effect on the antioxidant activity indicated seed priming with 1 mM concentration in 24 hours, leading to 84 and 62.6 increment in the catalase (CAT) and peroxidase (POD) activities, respectively, compared to the control. Furthermore, 0.1 mM concentration in 24 hours led to 61.2 and 66.2 increment in chlorophyll a and total chlorophyll, respectively.