Organogenesis from Callus Culture of Haplophyllum virgatum var. virgatum

Somayeh Torabi | Farah Karimi | Morteza Yousefzadi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=116493
Date :  2019/04/24
Publish in :    هشتمين کنگره ملي گياهان دارويي


Keywords :Organogenesis, Haplophyllum, virgatum, virgatum

Abstract :

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The genus Haplophyllum (Rutaceae) grows naturally around the Mediterranean section of Europe and in Western Asia up to Siberia and have about 70 species worldwide 1. Iran with 26 Haplophyllum species out of which 14 are endemic, has the highest number of this genus and can be considered as a center of speciation of this genus 2. The common Persian name of the genus is “Sodaby” 3. Many species of this genus are used in traditional medicine for the treatment of herpes, warts, stomachache, erysipelas, toothache, skin diseases 4 and in the treatment of testicular cancer 5. The presence of essential oils, alkaloids, fixed oils, coumarins, sterols, flavonoids and lignans have been reported from different species of the genus. Plant tissue culture is a technique of in vitro cultivation of plant cells and organs, which divide and regenerate into whole plants or organs and produced new and high medicinal plants at short time. In the present study regeneration in vitro conditions were investigated in callus from different seedling explants (leaf, shoot and root). Callus was induced on B5 medium supplemented with kinetin (0.1, 0.2 and 0.5 mg/L) in combination with NAA and IAA (1, 2 and 5 mg/L) then incubated in darkness at 25°C. The best results of callus fresh weight were obtained when shoot explants cultured on B5 medium supplemented with 0.1, 0.2 mg/L Kin and 5 mg/L NAA after two months. Formation of shoots induced from callus by culturing on B5 medium containing 1, 2 mg/L BA and Kin. Different shoot regeneration percentages were observed in employed PGR treatments in cultured explants after two months. This is the first report of in vitro culture of H. virgatum var. virgatum as an Iranian endemic plant.