Effect of surgical preiodondatal treatment on levels of immunologic factors in periodontitis patients

Shahrzad Shahbeik | Ferial Taleghani | Mandana Sattari

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=127036
Date :  2019/10/11
Publish in :    نوزدهمين کنگره بين المللي انجمن علمي پريودنتولوژي ايران


Keywords :Chronic periodontitis, priodontal phase I treatment, surgrical intervention, IL-18, NLRP3.

Abstract :

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Background. Periodontitis is a multifactorial inflammatory disease, which activated innate and adaptive immune system and causes to teeth supporting tissues destruction. Progress of periodontal disease is associated with the interaction between periopathogenic bacteria and immune system cells such as IL-1β and IL-18. IL-1β production is regulated by NLRP3 which is the member of NOD-like receptors (NOD) proteins. IL-18 is inflammatory cytokine. There is significant relationship between periodontal disease severity and IL-18 Methods. In this exprimental study, 18 patients (9 males and 9 femals) with the mean age of 46.2 years old were selected among patients with chronic periodontitis. GCF was collected before treatment, 4 weeks after phase I treatment and 4-week after phase II treatment by perio-paper. The concentration of NLRP3 and IL-18 were determined by ELISA method. Paired t-test was used for compression of NLRP3 and IL-18 concentration before and after treatment with CAL and PD. Results. The results have shown a significant relationship between pre-treatment mean PD and mean CAL and NLRP3 concentration (P =0.000). After each treatment phase, a significant decrease in NLRP3 concentration was revealed. However, there is no significant relationship between IL-18 and NLRP3 concentration (pre and post treatment and surgical intervention) with clinical parameters (mean PD, mean CAL in pre and post treatment and surgical intervention). Conclusions. Probably due to the correlation between NLRP3 concentration and clinical parameters, it can be considered as an index of inflammation in chronic periodontitis. Due to the significant reduction in NLRP3 concentration after each treatment phase, it can be used as an index for treatment evaluation in patients with periodontitis.