Shahed University

A New Approach for Determining the Minimum Concentration of Proanthocyanidin for Preservation of Collagen in H Dentin ABSTRACT Purpose:

Azadeh balalaie | Mohamad Rezvani | Mahshid Basir | Hassan rezadoost | Saeed balalaie

Date :  2019/07/10
Publish in :    European Journal of Prosthodontics and Restorative Dentistry

Keywords :Collagen Dentin Matrix Metalloproteinase Proanthocyanidin Maldi-Tof Mass Spectrometry

Abstract :
Purpose: Introducing a minimum concentration and clinically relevant application time for grape seed extract (GSE) proanthocyanidin as a dentin preserver. Materials and Methods: Dentin beams were demineralized in 10 phosphoric acid for 24h. Then, the following groups were prepared: G1: no treatment, G2: 2 CHX + 1min, G3: 1 GSE + 1min, G4: 1 GSE +5min, G5: 2 GSE + 1min, G6: 2 GSE + 5min, G7: 5 GSE + 1min and G8: 5 GSE + 5min. After 30 days, MALDI-TOF mass spectrometry was used to confirm the availability of digested peptide fragments and monitor the pattern of collagen digestion. Gravimetric assessment and HPLC-UV were utilized for quantitative measurement of dentin destruction. Glycine quantities were considered as measures of collagen digestion. Results: 7 to 25 loss of dry mass was measured in experimental groups. Regarding the liberated Glycine, GSE dose- and time-responses were observed, so that, 5 GSE showed considerable protecting effect on collagen compared to 1 and 2 GSE (P0.001) and 5min GSE application could establish superior dentin preservation compared to 1min application (P0.001). Conclusion: 5-minute treatment of dentin at 2 GSE and above is essential for protecting the demineralized dentin collagen against biodegradation.