The effect of nanozirconia mixed with glass-ionomer on proliferation of epithelial cells and adhesive molecules

Hasan Semyari | Maedeh Pournasir4 | Mandana Sattari | Mohammad Atai

---

URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137198
Date :  2012/08/01
Publish in :    Journal of Periodontology & Implant Dentistry- تغيير عنوان يافته


Keywords :Epithelium, glass-ionomer, ICAM-1, zirconia.

Abstract :

---

Background and aims. The aim of the present study was to evaluate the effect of nanoparticles of zirconia mixed with glass-ionomer on the proliferation of epithelial cells and adhesive molecules (ICAM-1). Materials and methods. Zirconia nanoparticles were mixed with glass-ionomer powder in weight percentages of 0, 5, 50, 70, and 100. The powders were then mixed with glass-ionomer liquid in 2: 1 weight ratios. The paste was then inserted into a steel ring mold (5 mm in diameter and 0.5 mm in thickness) sandwiched between two glass slides. Glass-ionomer was then cured using a light-curing unit. Seven samples (discs) were prepared for each mixing percentage. Cell cultivation (epithelial) and MTT tests were performed to assess the cytotoxicity of specimens containing different nanozirconia contents. Finally, human ICAM-1 platinum ELISA test was performed for quantitative diagnosis of human ICAM-1 epithelial cells.