Antibacterial activity of Nigella sativa ethanolic extract against two pathogenic bacteria

Mohammad Mehdi Attarpour Yazdi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137405
Date :  2019/01/17
Publish in :    11th International Congress of Laboratory and Clinic


Keywords :Keywords: Staphylococcus aureus, Salmonella typhimurium, Antibacterial activity, Nigella sativa,

Abstract :

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Background: Medical herbs with anti- microbial activity have always been important in traditional medicine and might be useful in antibacterial activity against the many pathogenic bacteria cause serious infections. The aim of this study was to determine the antibacterial activity of ethanolic extract from Nigella sativa against 2 pathogenic bacteria in vitro. Methods: At first the seed of Nigella sativa extracts were prepared by the ethanolic wetting extraction method in five different concentrations (400, 200, 100, 50, 25µg) and then its antibacterial activity against 2 standard strain bacteria (Staphylococcus aureus and Salmonella typhimurium) was tested for the determination of MIC (minimum inhibitory concentration) and inhibitory zone diameter (ZOI) using disk diffusion method and agar serial dilution assays. Also the antibacterial activity of 15 antibiotics such as chloramphenicol, trimethoprim, streptomycin, gentamicin, erythromycin, doxycycline, nalidixic acid, ampicillin, ciprofloxacin, kanamycin, cefixime, and methicillin was tested by the disk diffusion method. Results: Statistical methods were using to analyze the data. The results demonstrated that the Nigella sativa ethanolic extracts been effective against the one of the 2 standard strain bacteria (Staphylococcus aureus) and no effective against Salmonella typhimurium. For Staphylococcus aureus the ethanolic extract of Nigella sativa was highly effective with 500 µg concentration and exhibited greater antibacterial activity than all of the selective antibiotics. Conclusion: This study demonstrates that ethanolic extracts from the combination of the Nigella sativa constituents have excellent antibacterial activity against the Staphylococcus aureus. Further investigations will be necessary.