The effect of nobiletin on inflammatory response, oxidative stress, and apoptosis in a model of Parkinson’s disease induced by intranigral injection of lipopolysaccharide in the rat

Zahra Kiasalari Reineh | Sedigheh Keshtkar | Maryam Khorasani | Marzieh Fakour | Reihane Ghasemi | Mehrdad Roghani

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137489
Date :  2019/10/30
Publish in :    24 امين کنگره ملي و سومين کنگره بين المللي فيزيولوژي و فارماکولوژي ايران


Keywords :Nobiletin, Oxidative stress, Inflammation, Apoptosis, Parkinsons disease, Lipopolysaccharide

Abstract :

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Background and Objective: Nobiletin is a flavonoid in citrus. The antioxidant, anti-inflammatory and anti-apoptotic properties of nobiletin have been observed. The aim of the present study was to investigate the effect of nobiletin (NOB) on inflammatory response, oxidative stress and apoptosis in an experimental model of Parkinsons disease (PD) induced by intranigral injection of lipopolysaccharide (LPS) in rats. Materials and Methods: Male rats (n = 28) were divided into four groups: sham, sham under treatment of NOB, lesion and lesion treated with NOB. To achieve unilateral lesion of the nigrostriatal system, rats received 5 μg of LPS into the right substantia nigra. NOB was administered p.o. at a dose of 10 mg/kg/day from one hour after surgery till one week later. One week post-surgery, oxidative stress markers, inflammatory factors, and severity of apoptosis were measured. Results: The results showed that treatment with NOB significantly decreased malondialdehyde (MDA), reactive oxygen species (ROS), increased glutathione (GSH), superoxide dismutase (SOD) activity, decreased inflammatory factors including nuclear factor-kappa, Toll-like receptor 4, tumor necrosis factor and also decreased DNA fragmentation in LPS group. Conclusion: Administration of NOB to LPS-induced PD reduces oxidative stress, inflammation and apoptosis, which may potentially be of benefit for ancillary therapy of PD.