The effect of fenugreek methanolic extract on learning and memory in experimental model of Alzheimer’s disease in rat

Leila Nasiri | Hoora Zardooz | Zahra Kiasalari Reineh | Mehrdad Roghani

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137491
Date :  2019/10/30
Publish in :    24 امين کنگره ملي و سومين کنگره بين المللي فيزيولوژي و فارماکولوژي ايران


Keywords :Alzheimers disease, Trigonellafoenum-graecum, Amyloid beta, Learning and memory

Abstract :

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Background and Objective:Alzheimers disease (AD) is regarded as the most prevalent phenotype of dementia with debilitating disability in learning and memory. With respect to anti-oxidative and neuroprotective potential of Trigonellafoenum-graecum (TFG; fenugreek), this study was conducted to evaluate the effect of its alcoholic seed extract on learning and spatial memory in an experimental model of AD induced by amyloid beta 25-35 in rats. Materials and Methods: In this experimental study, 32 male Wistar rats were divided into 4 groups, i.e. sham, extract-treated sham, AD, and AD group treated with TFGextract. For induction of AD, β-amyloid 25-35 (at a dose of 10 μg/2 μl) was bilaterally microinjected into CA1 area of the hippocampus. The treatment groups received alcoholic extract of TFG (i.p.) at a dose of 200 mg/kg for 1 week till 1 h before the surgery. At 4th week postsurgery, learning and memory was assessed using passive avoidance test and spatial memory was evaluated in Y maze. Results: Treatment of AD group with the extract significantly prevented the reduction of step-through latency and extract treatment had no significant effect on spatial memory. In addition, extract treatment did not have a significant effect in the sham group. Conclusion: TFG extract pretreatment of Alzheimeric rats could improve the ability of information consolidation and retrieval in passive avoidance test, however it could not affect recognitive spatial memory.