THE CONSIDERATIONS AND THE REQUIRED STEPS FOR PRODUCTION OF THE PLANT SEEDS VACCINES WITH FOCUS ON THE ORAL DELIVERY

MAHDI YONESI | Alireza Rezazadeh | MELINTIANA MARIA MOUSAI

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137501
Date :  2020/03/24
Publish in :    Plant Cell Biotechnology and Molecular Biology

Link :  http://www.ikprress.org/index.php/PCBMB/article/view/4981
Keywords :Recombinant vaccine, oral delivery system, transgenic seeds.

Abstract :

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The recombinant vaccinal proteins can provide a solution for prevention of the contagious diseases, especially in deprived countries. While using eukaryotic platforms such as E. coli lack the post-translational modifications for expression of the proteins and using mammalian cells and plant cells encounter a labor-intensive and continuous process of cell culture and the final product requires special transportation logistics, the plant seeds can provide a good solution for expression of the vaccinal proteins as an oral vaccine. Seeds contain a low amount of water content and can show a considerable bioencapsulation to protect the recombinant products from environmental degradation. However, for the expression of a recombinant protein, there are steps and considerations that should be considered. These considerations include the choice of an appropriate seed to be used as an expression platform (bioreactor), the choice of the promoter, adjustment of the codon preference, targeting the protein for in the cell and finally purification of the vaccinal protein in case of need. This article aims to describe the details of the recombinant vaccine production in the seed platform with the focus on oral delivery of the vaccine at a glance.