Effect of priming on seed germination and morpho-physiological traits of Portulaca oleracea L. under salinity stress

Z Hassani | N Amraee | K Ahmadi | Heshmat Omidi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137618
Date :  2021/05/18
Publish in :    علوم و تحقيقات بذر ايران


Keywords :Anthocyanin, Proline, Portulaca oleracea, Chlorophyll, Sodium chloride, Soluble sugars

Abstract :

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Salt tolerance at the germination stage is important, because salinity is usually severe at the soil surface. Especially in cases where the groundwater level is high. In order to investigate the effect of priming and salinity stress on the quantitative and qualitative traits of P. oleracea, this experiment was conducted in the Seed Technology Laboratory of the Faculty of Agricultural Sciences of Shahed University in 2019. Experimental agents included pretreatments (control (distilled water), hydro-priming, potassium nitrate and salicylic acid) in response to 6 salinity levels (no salinity as control and salinity of 30, 60, 90, 120 and 200 mM with Sodium Chloride). This experiment was studied as a factorial in a completely randomized design with three replications. The measured traits included germination indices, photosynthetic pigments, anthocyanin content, proline and soluble sugars. The results showed that the highest amount of germination components such as germination percentage (97), germination rate (2.16 seeds per day), plantlet length (6.69 cm), plantlet fresh weight (4 g), plantlet length index (324.69) and RWC (75.25) were obtained in non-salinity stress and application of hydro-priming pretreatment. At salinity levels of 60 mM, the content of chlorophyll a, chlorophyll b and total chlorophyll in the hydropriming treatment increased and the highest content of proline, soluble sugars and anthocyanin was obtained at the highest salinity level. Among the studied priming levels, hydropriming at salinity stress of 200 mM had the highest percentage and seed germination rate, chlorophyll content, anthocyanin and proline.