Association of dietary intake of fruit and green vegetables with PTEN and P53 mRNA gene expression in visceral and subcutaneous adipose tissues of obese and non-obese adults

Golnoosh Kadkhoda | Maryam Zarkesh | Atoosa Saidpour | Masoumeh Hajizadeh Oghaz | Mehdi Hedayati | Alireza Khlaj

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=137930
Date :  2020/04/05
Publish in :    Gene
DOI :  https://doi.org/10.1016/j.gene.2020.144353
Link :  http://dx.doi.org/10.1016/j.gene.2020.144353
Keywords :Fruit Vegetables P53 PTEN Gene expression Obesity

Abstract :

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Objective: The present study investigates the association of dietary intake of fruit and green Vegetables with PTEN and P53 mRNA gene expression in visceral (VAT) and subcutaneous adipose tissues (SAT) of obese and non-obese adults. Methods: VAT and SAT were obtained from 151 individuals, aged ~40 years, who had undergone elective abdominal surgery. The participants were grouped according to their body mass index (BMI), as obese (BMI 30 kg/m2) and non-obese (BMI = 18.5–30 kg/m2). Dietary intakes were obtained using a valid and reliable food-frequency questionnaire (FFQ). Real-time PCR was carried out for PTEN and P53 mRNA expressions. Associations between expression levels and dietary parameters were analyzed. Results: P53 mRNA expression of obese participants was significantly higher than the non-obese, only in VAT (p 0.001). After adjusting for total energy intake, age and BMI, fruit intakewas inversely associated with P53 gene expression in both VAT (β = −0.38, P = 0.01) and SAT (β = −0.35, P = 0.03) among non-obese participants. Furthermore, fruit consumption was inversely associated with P53 gene expression in obese individuals, only in VAT (β = −0.21, P = 0.05). More so, intake of green vegetables in obese subjects was negatively associated with P53 gene expression in VAT (β = −0.27, P = 0.01) and SAT (β = −0.28, P 0.001). Onthe otherhand,afteradjustment fortotal energyintake, age andBMI,a positiveassociation was observed between fruit intake and PTEN in VAT (β = 0.27, P = 0.01) and SAT (β = 0.34, P 0.001) among obese participants. In addition, dietary consumption of fruits in non-obese individuals was negatively associated withPTEN expression in SAT (β = −0.48, P 0.001). Conclusion: Dietary intake of fruit and green vegetables was associated with P53 gene expression in VAT and SAT of obese participants, suggesting their protective role in regulating P53 mRNA expression in adipose tissue. Furthermore, higher fruit intake was inversely associated with PTEN mRNA levels in non-obese participants, implying the anti-adipogenic role of PTEN gene expression.