Injection of colchicine into the dorsal striatum of rats brain induces epilepsy

Manizheh Karami | Mehrdad Roghani | Atiyeh Nejadebrahim | Zahra Fakhroleslam

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=148254
Date :  2020/12/21
Publish in :    Journal of Basic and Clinical Pathophysiology

Link :  http://jbcp.shahed.ac.ir
Keywords :Generalized epilepsy, Dorsal striatum, Identified doses, Colchicine, Wistar rat

Abstract :

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Abstract Background and Objective: Despite the massive use of costly models, our understanding of epilepsy is insignificant. We aimed to create the cost-beneficial and applicable generalized epilepsy (GE) in rats by injection of identified doses of colchicine (IDC) into the dorsal striatum of rats brain. Materials and Methods: 24 male Wistar rats (290-350 g) under deep anesthesia were equipped with guide cannulae at the dorsal striatum (AP: 0.48 mm; L: 3 mm; V: 3.6). The injection cannula attached with the polyethylene tubing to the 5-μL Hamilton syringe guided daily IDC (9-25 μg,) into the dorsal striatum of rats brain during four consecutive days. The control group only received the saline solution. At the end of the injection, the animals behavior was investigated. Finally, all the brains were collected in 10 formalin solution and dissected out to include the striatal regions. The samples were cut coronally into 3-4 μm thick slices, embedded in paraffin, and processed by the step section technique. The slices were then stained with Hematoxylin-Eosin method and checked under the light photomicroscope for correct placement of injections as well as the possibility of lesions. The data were compared between vehicle and experimental groups by analysis of variance (ANOVA). Results: The findings show that GE occurs in animals receiving colchicine without significant devastation effect in the injection site in the brain of rat. Conclusion: The plant-derived alkaloid, the colchicine, aside depolymerizing of tubulin, may have an inductive effect on epilepsy in the complementary motor striatal region of the rats brain