Changes in antioxidant enzymes activities and alkaloid amount of Catharanthus roseus in response to plant growth regulators under drought condition

M Ababaf | M Bakhshande | Heshmat Omidi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=158456
Date :  2021/09/19
Publish in :    Industrial Crops and Products

Link :  https://www.sciencedirect.com/science/article/abs/pii/S0926669021002697?via3Dihub
Keywords :Catharanthus roseus, Drought, Medicine plant, Oxidative stress, Plant growth

Abstract :

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The aim of this study was the evaluation of the influence of salicylic acid (SA) and jasmonic acid (JA) on some antioxidant and alkaloids responses of Catharanthus roseus to drought stress on the farm. Accordingly, a two-year field experiment was a split-plot conducted in 2016–2017 with three drought levels control 40 of available soil water was depleted (MAD) = as control, 60 and 80 MAD, and eight-level SA and JA foliar (0, 0.1, 1 and 10 mM SA) and (0 and 10 µM JA) were tested as the experimental treatments. Results indicated that the studied traits improved by the SA spraying. Total fresh and dry weights and antioxidant enzymes activity in treatment 10 mM SA and relative water content, contents of chlorophyll-a, b, total and carotenoid in 1 mM SA concentration gave better result than other SA concentrations. Foliar application of JA treatment decreased contents of chlorophyll-a, b, total and carotenoid under non-stressed and stress conditions. Application SA alone and 0.1 mM SA+10 µM JA increased TFW and TDW in the plants were under stress condition. Also, the antioxidant enzymes activity included peroxidase (POD), ascorbate peroxidase (APOX), superoxide dismutase (SOD) and polyphenol oxidase (PPO) enhanced by spraying all concentrations of SA, except 10 mM, with the 10 µM JA, application JA alone and with 0.1 mM SA increased the antioxidant enzymes activity. The interesting point about this research study is the increased production of secondary metabolites (i.e. phenolic compounds) under stress, affecting Catharanthus response and medicinal properties. Accordingly, it may be possible to adjust the production of secondary metabolites (medicinal contents) in Catharanthus by adjusting the irrigating practices.