Effect of gamma irradiation on cytotoxicity, phenolics content and acute toxicity of Cuscuta chinensis L. extract

Fatemeh Alijaniha | Fatemeh Emadi | Mohsen Naseri | Mohammad Kamalinejad | Elahe Motevaseli | Malihe soodi | Roya Karimi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=158934
Date :  2021/04/29
Publish in :    Radiation Physics and Chemistry: the journal for radiation physics, radiation chemistry and radiation processing
DOI :  https://doi.org/10.1016/j.radphyschem.2021.109508
Link :  http://dx.doi.org/10.1016/j.radphyschem.2021.109508
Keywords :Gamma irradiation Cuscuta chinensis L. Phenolics content Cytotoxic effect Acute oral toxicity

Abstract :

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Gamma irradiation is used as an efficient method for decontamination of some foodstuffs and herbal medicines. Several studies have shown that low to moderate doses of gamma irradiation do not alter the chemical composition and biological activities of herbal medicine; however, some changes in herbal composition, appearance or properties have been observed in another studies, and this remains controversial. The present study aimed to evaluate the effect of gamma irradiation on the biological activity and the content of bioactive compounds of Cuscuta chinensis L. extract. The dose of 5 kGy was studied for decontamination. The results of the in vitro study showed that the cytotoxic effects on A549 and HT-29 cell lines were improved without any alteration of cytotoxicity on normal cell line. Also, acute oral toxicity of the extract did not change according to in vivo study. However, due to the significant reduction of total phenol and flavonoids contents by 12 and 18 respectively, the improvement of cytotoxic properties maybe due to the formation of new active compounds, the identification of which requires further studies.