Superparamagnetic Iron Oxide Nanoparticles Modified with Alanine and Leucine for Biomedical Applications: Development of a Novel Efficient Preparation Method

Karimzadeh         Isa | Mustafa Aghazadeh | Davoud Gharailou | pirhossein kolivand | Taher Doroudi | Mohammad Reza Ganjali

---

URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=159040
Date :  2017/06/01
Publish in :    Current Nanoscience

Link :  10.2174/1573413713666170118 125937
Keywords :Superparamagnetic nanoparticles; alanine; amino acid coat; biomaterials; cathodic electrosynthesis; leucine; magnetite; surface coating

Abstract :

---

Background: Magnetic nanoparticles (MNPs) are interesting candidates for biomedical areas of cell targeting and separation, magnetic resonance imaging (MRI), cancer therapy, hyperthermia, gene and drug delivery. Up now, various kind of surface coatings like polymers, polysaccharides, organic acids, silica and amino acids. The type of surface coating and their geometric connection onto the surface of MNPs determine the overall size, surface charge, magnetic characters, cytoxicity, bio-distribution, bio-compatibility and bio-performance. Amino acids can be interesting coating candidates for magnetite NPs because of their excellent bio-compatibility, stabilization and important role in the body. Hence, there has been increasing interest concerning amino acid coatings of MNPs. Methods: Uncoated MNPs were electrodeposited by applying the current density of 10 mA/cm2. The bath temperature and deposition time were 25 °C and 30 min, respectively. Then, the black deposit was scraped from the surface of steel electrode and then subjected to the purification process. For preparation of AMINO acid coated MNPs, 1g/L amino acids (alanine or leucine) were only added into the 0.01 M FeCl2/Fe(NO3)3 solution. The purification steps were done for the prepared uncoated MNPs, alanine- and leucine coated MNPs. The obtained black powders were labeled amino acid-coated NPs (i.e. bare NPs, alanine- and leucine- coated- NPs) and evaluated by characterization analyses. Results: The position and relative intensity of all XRD diffraction peaks are well-matched with a cubic spinel structure of magnetite (i.e. Fe3O4, JCPDS 01-088-0315). High magnification by TEM revealed that the prepared MNPs have size about 10 nm. It was found that the decrease in size and low agglomeration are due to the amino acid coating, which prevents agglomeration of the Fe3O4 fine particles during their deposition and growth on the cathode surface and hence reduces the size of the nanoparticles. The IR data completely specified the presence of the alanine and leucine on the surface of the electro-synthesized MNPs. VSM data revealed the amino acid coated NPs have very negligible Mr and Ce values, which indicated their better magnetic behavior as compared with naked NPs.