Effectiveness of Fe2O3 nanoparticles more than magnetic field against the destructive effect of colchicine on Paramecium caudatu

Manizheh Karami | Matineh Sheikhmohammadi | Abazar Hajnorouzi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=159567
Date :  2022/06/26
Publish in :    Journal of Basic and Clinical Pathophysiology

Link :  http://jbcp.shahed.ac.ir
Keywords :Keywords: Paramecium caudatum, Colchicine, Static magnetic field, Fe2O3 nanoparticles

Abstract :

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Abstract Background and Objective: Colchicine depolymerizes microtubules and reduces their diamagnetic capacity. We investigated the effect of a static magnetic field (SMF) and the Fe2O3 NPs on the Paramecium caudatum exposed to colchicine. Materials and Methods: The animals were collected from temporary water sources, and after identifying the species, were cultured in the laboratory. They were also sub-cultured at regular intervals (7-10 days) for purification. From pure culture, several samples were placed in the laboratory or under SMF with an intensity of 61 mT for 3 days. To evaluate the effect of materials in comparison with distilled water (control), a sample (0.1 mL) of each medium was placed on a slide and exposed to 1 μL of distilled water or volume-concentrations of colchicine (0.05 to 25 μg/μL) or Fe2O3 NPs (0.05 to 3 μg/μL). The effect of different concentrations of Fe2O3 NPs in accompany with the field was also investigated. The movement of the animal was examined for 30 sec under a constant view and the sample was fixed for staining and study at the cellular level. Data were analyzed using analysis of variance. Results: Colchicine at high concentrations (15 and 25 μg/μL) significantly reduced the motility of Paramecium caudatum. Magnetic field, but not NPs, alone reduced cell motility. Co-exposure to particles completely improved cell motility due to exposure to colchicine, however, in animals initially housed in the magnetic field, the healing effect of the particles was impaired. Conclusion: The protective effects of NPs may depend on the diamagnetic capacity of the microtubule.