Dose-dependent effect of Hypericum perforatum extract on motor imbalance following intrastriatal injection of 6-hydroxydopamine in the rat

Mohsen Khalili | Batool Rahmati | Mehrdad Roghani | Gholamhosein Ghaedi | Siamak Afshinmajd | Zahra Kiasalari Reineh | Manizheh Karami

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=31745
Date :  2016/03/10
Publish in :    Journal of Basic and Clinical Pathophysiology


Keywords :Hypericum perforatum 6-hydroxydopamine Rotational behavior Motor imbalance Dose-dependent

Abstract :

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Background and Objective: Parkinsons disease (PD) is a neurodegenerative disease with selective damage of mesencephalic dopaminergic neurons. Due to the protective, anti-inflammatory, and antioxidant effect of Hypericum perforatum (HP), this study was undertaken to assess dose-dependent effect of HP hydroalcoholic extract on motor imbalance following intrastriatal injection of 6- hydroxydopamine in the rat. Materials and Methods: In this experimental research, male Wistar rats (n=35) were equally divided into sham, 6-hydroxydopamine (OHDA)-lesioned, and HP extract-treated lesion groups. Model of PD was induced by microinjecting 12.5 microgram of 6-OHDA dissolved in saline-ascorbate solution into the left striatum. Treated lesion groups received HP extract at doses of 50, 100, and 200 mg/kg/day p.o. started one week before the surgery for 1 week post-surgery. After 1 week, ipsilateral and contralateral rotations induced by apomorphine were counted and net scores were obtained. Results: In the 6-OHDA-lesioned group, the dopaminergic agonist apomorphine induced contralateral rotational behavior (P0.001) as compared to sham. In addition, administration of HP extract at doses of 100 and 200 mg/kg significantly reduced the number of contralateral rotations (P0.05) versus 6-OHDA group. Conclusion: Oral administration of HP extract at doses of 100 and 200 mg/kg could reduce motor imbalance and attenuate forced biased rotational behavior in 6- OHDA-induced model of PD.