Test of chemical toxicity of a neurotoxin...Paramecium caudatum

Rababeh Mohammadi          Manizheh Karami

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=42265
Date :  2016/08/23
Publish in :    17th international and Iranian congress of Microbiology


Keywords :Paramecium caudatum, Sevage water, Chemical toxicity, colchicine

Abstract :

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Background: Chemical toxicity of savage water living microorganisms has been shown in literatures. But, no evidence indicates the toxicity of a microtubule element degrader, colchicine, in the Paramecium caudatum. Objective: This research evaluated the response of P. caudatum to the toxin colchicine. Materials & Methods: The organisms were isolated from savage water sources around the Shahed University main campus in Khalij-e-Fars highway and cultivated at the laboratory. After preparing the pure cultivation of the organisms 0.1 mL of the cultivation was added in the slides. The drug in a volume of 0.01 - 1μg/μL was infused into the cell drop, at a defined point of the field provided by 4X of the photomicroscopy. The microorganisms’ movements were recorded as count per 60 sec considering the 5 sec interval times’ points. All data were also recorded using the video- and photo-microscopy. The data further were analyzed using the compare of means program. Results: The Paramecia locomotors activities under the infusion of toxin were not significantly affected at the point of the drug infusion. The most effect (dispersal of the organisms) was observed at a relatively high doses of the drug (0.1 - 1 μg) at a significant level (p0.05). Discussion and Conclusion: Toxicity by chemical substances in the savage water living microorganisms may rarely be shown. The dispersal of the P. caudatum may provide a sign to show the chemical toxicity by materials with no effect on the cells’ activities at the cellular and molecular levels.