Aloe Vera’s Effect on Acetyl Cholinesterase(AChE) and Ultra Structure of Motoneurons in Synaptic Zone After Spinal Cord Injury in Rats

Marjan Heshmati | Mohammad Reza Jalali | Nadoushan Nadoushan | Maryam Sharayeli

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=42280
Date :  2016/06/15
Publish in :    Biosciences Biotechnology Research Asia
DOI :  https://doi.org/10.13005/bbra/2119
Link :  http://www.biotech-asia.org/vol13no2/aloe-veras-effect-on-acetyl-cholinesteraseache-and-ultra-structure-of-motoneurons-in-synaptic-zone-after-spinal-cord-injury-in-rats/
Keywords :Ultra, Structure, Motoneurons, Synaptic, Spinal

Abstract :

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Spinal cord injury(SCI) followed by transection, contusion or compression areconsidered to be similar to human SCI.Nowadays researchers demonstrated alternativetherapies as pharmacological properties or herbs to repaire these damage.Aloe Vera is known as a plant with multi potential activity.We used this plant after SCI by Clips Aneurysm(30g /1 min). 32 female Sprague Dawley rats were divided randomly into four groups.Group 1: Sham( laminectomy) + gavage Distilled water, group2: Sham +gavage Aloe Vera gel powder(200mg/kg/d), group3: group 2+SCI, and group4:group 1+SCI (N=8).After 4 weeks they were sacrificed.Morphometric study and Acetyl cholinesterase(AChE) immune staining were done.Synaptic changes was analyzed in a blinded manner for qualitative ultra structural changes.The data analyzes with Tukey’s test and one-way ANOVA in SPSS21 software and P≤0.05 was considered as significant level.Decreasing motoneurons ,reducing AChE immune activity with ultrastructure changes in mitochondria and synaptic zone were seen due to SCI.Usage of Aloe Vera gel powder demonstrated a reduction in death of motoneuron(Pd”0.05) with increasing AChE immunoreactivity after SCI(Pd”0.05).This herb affected on ultrastructure as seen pathological synaptic changes and mitochondria decreases.Aloe Vera gel powder effects might be due to it’s antioxidant which has reduced neuronal cell death and preserved damaged neurons as intact neurons.