Immunomodulatory Effects of some Allium species from Iran on mice Lymphocyte viability

Hosseinpur Z | Tayebeh Radjabian | Tooba Ghazanfari | Zarre-Mobarakeh Sh | Soleymankhani M | Mirsharif E | Dilmaghanian R

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=43678
Date :  2016/04/29
Publish in :    13th International Congress of Immunology and Allergy of Iran

Link :  www.icia.ir
Keywords :Bulb extract, Allium species, Lymphocyte viability

Abstract :

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Introduction: Identification and application of immuno-modulators in natural ingredients can be effective in immune regulation. Lymphocytes are the cell components of adaptive immune system. In the present study, the aqueous extract effects of selective Allium species on viability of these cells were examined. Materials and Methods: Fresh bulbs of seven wild Allium species were collected from their natural habitats and the bulbs of cultivated A. sativum were supplied from a field in Hamadan. Aqueous extracts of the fresh bulbs were prepared. Lymphocytes population was prepared from mice spleen. The acquired cell pellet was cultured in RPMI/FBS.Different concentrations of aqueous extracts were added to the cell cultures, and cell viability was measured by MTT assay. Results: Based on the obtained results, significant differences (P0.05) were observed among the aqueous extracts of Allium species in some concentrations on viability of lymphocytes. The results showed that bulb extracts of A. sativum at 0.001 and 0.0001, A. jesdianum at 0.05, 0.001 and 0.0001, A. lenkoranicum at 0.1, 0.05, 0.005, 0.001 and 0.0001, and A. stipitatum at 0.1 mg/mL had stimulatory effects and A. Iranicum at 1 and A. elburzens at 1 mg/mL had inhibitory effects on viability of lymphocytes.Conclusion:Our findings approved that the bulb extracts of all the examined Allium species had stimulatory or inhibitory effects on viability of lymphocytes in different concentrations, among them the best stimulatory results were obtained for A. lenkoranicum, followed by A. jesdianum and A. sativum in low concentrations.

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