The effect of inhibition of opioid receptors by naloxone on cyst genesis of morphine intra-VMH

Mina Ghaffari | Manizheh Karami

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=43857
Date :  2017/05/20
Publish in :    The 3rd International Congress on Reproduction-ISERB 2017


Keywords :Morphine, naloxone, polycystic ovarian syndrome, Rats

Abstract :

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Introduction The authors today know the complication Ovarian Polycystic a cause of infertility; that drugs including morphine have been known to play effective role in the incidence of this complication, too. Naloxone is known a competitive inhibitor of morphine which connect with mu receptor at the central level, and the environment that has antagonistic effect on morphine. This study examined the interaction of morphine / naloxone in central hypothalamus of Wistar rats. Materials and Methods: Experimental animals were virgin female Wistar rats with Weight range of 250-200 g /. Morphine (0.001 to 0.4 micrograms / rat) was injected into ventro-medial hypothalamus of rat (nucleus coordinates: AP: -1.96). Naloxone (0.1 to 0.4 micrograms / rat) was injected alone or pre-microinjected. In the process of naloxone pre-injection, it (0.1 to 0.4 micrograms / rat) was administered prior to morphine (0.4 micrograms / rat). The control group received only saline. After completion of the experiments, the animals were anesthetized and after surgery, the ovaries were collected and investigated. Results: Naloxone intervention in group receiving naloxone in presence of morphine significantly reduced the number of cysts in the ovaries of rats. Ovarian aspect in the naloxone groups was as the control group. Conclusion: According to the results the interaction of morphine with opioid receptors in the nucleus of the hypothalamus is necessary in the development of polycystic ovaries, the complication that is stopped by inhibiting of morphine-receptor interaction due to naloxone intervention.