Changes in Gene Expression of P75 and Trk Receptors in Deprenyl-Treated Spinal Cord Injury Rats

Marjan Heshmati | Alireza Azizzadeh Delshad

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=7738
Date :  2013/02/01
Publish in :    World Applied Science Journal


Keywords :Changes, Expression, Spinal

Abstract :

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Abstract: Apoptotic cell death a prominent component of secondary events of spinal cord injury (SCI), may be influenced by neurotrophin receptors p75 and Trks which have been claimed both pro-survival and pro-apoptotic. In the present study to investigate the effects of the putative neuroprotectant, deprenyl, on the expression of neurotrophin receptors in SCI model, adult deprenyl treated rats were subjected to compressive SCI. After 2-72h the spinal cord tissues were processed to detect the p75NTR, TrkA and TrkB mRNA expression through semiquantitative RT-PCR. In the intact samples no expression of p75NTR occurred, whereas SCI induced a series of fluctuant changes and deprenyl put off its expression to 8h. In the SCI-groups the expression of TrkB receptor, changed in a contradictory manner and deprenyl caused an augmented overexpression of TrkB at 2h which tended to decrease in the following time points progressively. The expression of TrkA gene indicated no significant changes up to 4h following SCI, but thereafter its expression increased significantly and deprenyl treatment didnt induce any changes. It can be concluded that deprenyl can exert its neuroprotective effect through inhibiting the expression of p75NTR and upregulating the TrkB receptor in the early first hours following the injury.