Depressed Immune Responses and Accelerated Splenic Apoptosis due to Experience of Food Deprivation and Inequality but not Unstable Social Status in Balb/c Mice

Marjan Aghajani | Mohammadreza Vaezmahdavi | Mohsen Khalili | Tooba Ghazanfari | Fatemeh Moradi | Ravieh Golchoobian | Hasan Askari | Nima Sanadgol | Ehsan Kazemi Moghaddam

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=84590
Date :  2017/11/17
Publish in :    Neuroimmunomodulation
DOI :  https://doi.org/10.1159/000480732
Link :  https://www.karger.com/?DOI=10.1159/000480732
Keywords :Depressed, Responses, Accelerated, Inequality

Abstract :

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Abstract Objective(s): We aimed to show that the immune system is sensitive to the detrimental effects of inequality and social injustice, and splenic vulnerability to apoptosis may also increase. Methods: In order to better determine immune responses to chronic social stress, we implemented food deprivation, food intake inequality, and unstable social status (a change of cage-mate every 3 days) for a period of 14 days in 60 male Balb/c mice. At the end of this stress period, nitric oxide (NO) production by peritoneal adherent cells and the serum concentration of corticosterone were measured. Moreover, the viability of peritoneal adherent cells and spleen lymphocytes was evaluated by MTT assay. The terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay was done to reveal the TUNEL-reactive apoptotic bodies in the spleen. Results: Our results showed that food deprivation and inequality caused significant changes in the apoptosis of splenic cells in comparison with the control group ( p 0.05). Moreover, the vital activities of lymphocytes and peritoneal adherent cells, as well as NO production by the latter, increased significantly ( p 0.05). However, the experience of unstable social status did not cause a further increase in the viability of lymphocytes and peritoneal adherent cells, or NO production in animals that were fooddeprived or experienced inequality. Serum concentration of corticosterone in all experimental groups, except for animals. that experienced unstable social status only, significantly decreased versus the control group ( p 0.05). Conclusions: The results suggest that poverty and social inequality, but not unstable social status, affect immune responses and are likely involved in the induction of splenic apoptosis in mice.