Evaluation of anti-adenovirus activity of some plants from Lamiaceae family grown in Iran in cell culture

Horieh Saderi

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=85136
Date :  2018/05/12
Publish in :    هفتمين کنگره گياهان دارويي
DOI :  https://doi.org/10.5897/ajb10.1686

Keywords :Evaluation, Lamiaceae

Abstract :

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The family Lamiaceae has included some plants such as thyme species that in Iranian traditional medicine mentioned to have a lot of medical properties 1. Some of these properties have not approved by original article. The aim of this study was evaluation of anti-adenovirus effect of three plants from Lamiaceae family (Thymus daenensis, Thymus vulgaris, Zataria multiflora) that grown in Iran. The aqueous extracts of plants were prepared and the essential oil of Thymus vulgaris was purchased. Cytotoxicity effects of tested compounds were determined. The anti-adenovirus effects of maximum non-cytotoxic concentration of tested compounds were evaluated in cell culture with 100 TCID50 of adenovirus type 5 by three methods: simultaneous, pre-treatment and post-treatment. For antiviral effect only those concentrations were accepted that completely inhibit cytopathic effect of virus in cell culture 2. In simultaneous and post-treatment methods, minimum concentration that show anti-adenovirus activity were 12.5, 12.5, 25 and 50 µg/ml for Thymus daenensis, Thymus vulgaris, Zataria multiflora aqueous extracts and thyme essential oil, respectively. None of the tested compounds had shown anti-adenovirus effect in pre-treatment method. These results have suggested the potential use of these compounds for treatment of the adenovirus infections.