Differentiation of neurosphere-derived rat neural stem cells into oligodendrocyte-like cells by repressing PDGF-? and Olig2 with triiodothyronine

Hojjat-Allah Abbaszadeh | Taki Tiraihi | Alireza Delshad | Majid Sadeghizadeh | Taher Taheri | Hadi Kazemi | Hayder K. Hassoun

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URL :   http://research.shahed.ac.ir/WSR/WebPages/Report/PaperView.aspx?PaperID=9562
Date :  2014/12/01
Publish in :    Tissue and Cell
DOI :  https://doi.org/10.1016/j.tice.2014.08.003
Link :  https://www.ncbi.nlm.nih.gov/pubmed/25200619
Keywords :Differentiation, neurosphere-derived, neural, PDGF

Abstract :

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One of the approaches for treating demyelination diseases is cytotherapy, and adult stem cells are potential sources. In this investigation, we tried to increase the yield of oligodendrocyte-like cells (OLCs) by inducing neural stem cells generated from BMSCs-derived neurospheres, which were used for deriving the neural stem cells (NSCs). The latter were induced into OLCs by heregulin, PDGF-AA, bFGF and triiodothyronine (T3). The BMSCs, NS, NSCs and OLCs were characterized by using immunocytochemistry for fibronectin, CD44, CD90, CD45, Oct-4, O4, Olig2, O1 and MBP markers. PDGF receptor α (PDGFR-α), Olig2 and MOG expression were evaluated by RT-PCR. The BMSCs expressed CD44, CD90, CD106 and Oct-4; the NSCs were immunoreactive to nestin and neurofilament 68. Incubation of the NSCs for 4 days with heregulin, PDGF-AA and bFGF resulted in their induction into oligodendrocyte progenitor-like cells (OPLCs), which immunoreacted to O4, Olig2 and O1, while Olig2 and PDGFR-α were detected by RT-PCR. Replacing heregulin, PDGF-AA and bFGF with T3 for 6 days resulted in repression of O4, O1, Olig2 and PDGFR-α. The OLCs were co-cultured with motoneurons resulted in induction of MOG and MBP, which were expressed in functional OLCs. The latter can be generated from BMSCs-derive NS with high yield.